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Papers on Redback
Spiders
 [English]
[Japanese] |
| Original Paprer: HIRAOKA,T.; KOBAYASHI,M.; SADAHIRO,S.; AGUI,N.: Protein components and toxicity of venom gland-extract in the red back widow spiders, Latrodectus hasseltii collected in Osaka-City, Japan. 47(3):273-280 (1996) |
| Abstract |
_____Proteins in venom glands of Latrodectus
hasseltii collected in Osaka area were analyzed by sodium dodecylsulfate-polyacrylamide
gel electrophoresis (SDS-PAGE). Several main bands with molecular weight
of 110-120 kD were clearly recognized after protein staining. Around 45kD
area several distinct bands were also presented and another many bands
were presented from smaller than 20kD to greater than 200kD. Monoclonal
antibodies against  -latrotoxin
of L. tredecimguttatus bound to 110-120kD bands and also 45kD bands.
Lyophilized venom gland-extract imported from Australia showed a different
pattern of protein bands on SDS-PAGE gel, i.e. 110-120kD bands were not
clearly recognized as major bands and new band appeared around 77kD area.
From the result of binding of monoclonal antibodies to 77kD bands it was
suggested that these bands are degraded product of -latrotoxin._____All mice injected with venom gland-extract of one female spider died within 2 days after injection. Lyophilized venom gland-extract showed less toxicity than our preparation of venom glands of the red back spider collected in Osaka-City. Clear decrease of body weight of mice injected with the venom was observed during 2 days after injection. Present results clearly showed that red back spiders collected in Osaka had an -latrotoxin
in their venom glands and toxicity was not weak referring to previous data
of toxicity of widow spiders. |
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